Vol. 9, Special Issue 4, Part A (2023)

Abstract
The reactive oxygen species induces oxidation which can result in protein damage, mutations in DNA as well as disintegration of cell membrane. It can lead to induce development of various ailments like malignancy, cardiovascular and liver disorders, whereas antioxidants with free radical scavenging role, play a potential role in protecting damages by reactive oxygen species. In the current study, antioxidant activities of Clitoria ternatea, Guazuma ulmifolia and Madhuca indica were investigated. Their ethanolic extracts were determined based on their scavenging activity of the stable compound 1, 1-diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging assay. Crude ethanolic extract of C. ternatea, G. ulmifolia and M. indica were tested for their antioxidant activity by DPPH method spectrophotometrically and ascorbic acid was used as standard. Low absorbance is an indication of low concentration of free radicals and high antioxidant activity. The maximum antioxidant potential was seen in ethanolic extract of C. ternatea (84.00%) with the 500µg/ml concentration and minimal activity was observed in G. ulmifolia (62.67%). It is found that all the extracts have prominent antioxidant activities. The IC50’s (inhibition concentration 50) of the ethanolic extracts are Clitoria ternatea (3.93 μg/ml), Guazuma ulmifolia (5.88 μg/ml) and Madhuca indica (4.53 μg/ml). The results obtained in the present study indicate that Clitoria ternatea, Guazuma ulmifolia and Madhuca indica can be expected source of antioxidant agents. Therefore, the investigation clearly demonstrates that these three plants are exceptionally advantageous for human wellbeing.